“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy

“Humanized” Stem Cell Culture Techniques: The Animal Serum Controversy

Cellular therapy is reaching a pinnacle with an understanding of the potential of human mesenchymal stem cells (hMSCs) to regenerate damaged tissue in the body. The limited numbers of these hMSCs in currently identified sources, like bone marrow, adipose tissue, and so forth, bring forth the need for their in vitro culture/expansion. However, the extensive usage of supplements containing xenoge...

Basic Techniques in Animal Cell Culture

One of the characteristics of most animals is that they are multicellular−in other words− they are composed of many cells. With this multicellularity comes the specialization of cells. In a multicellular organism, each cell does not have to carry out all the activities necessary for the life of the organism. Although most cells of any higher organism have many organelles and metabolic pathways ...

Mesenchymal Stem Cell Purification from the Articular Cartilage Cell Culture

Objective Articular cartilage as an avascular skeletal tissue possesses limited capacity to heal. On the other hand, it is believed that the regeneration capacity of each tissue is largely related to its stem cell contents. Little is known about the presence of mesenchymal stem cells in articular cartilage tissue. This subject is investigated in the present study. Materials and Methods Artic...

Development of humanized culture medium with plant-derived serum replacement for human pluripotent stem cells.

For human embryonic stem cells (ESC) to be used in cell replacement therapies, they must be grown under good manufacturing conditions in a chemically defined medium that lacks animal proteins. This study examined the ability of a newly designed medium containing the plant-derived serum replacement VegetaCell and other reagents of human origin to support undifferentiated growth and pluripotency ...

Toward Animal Cell Culture